The gene Oryza sativa indoleacetic acid 13(OsIAA13) is involved in auxin signaling and controls the expression of genes that are required for lateral root initiation in rice. The open reading frame (ORF) length of OsIAA13 was 2116 bp. It's located on the long arm of chromosome 3.
A rice gain-of-function mutant, Osiaa13, contains a single amino acid substitution (G to A, which resulted in a single amino acid substitution, glycine (G) to serine (S), in the core sequence in domain II of OsIAA13) in the core sequence required for the degradation of the OsIAA13 protein. The Osiaa13 mutant displayed typical auxin-related phenotypes: the number of lateral roots was significantly reduced and the root gravitropic response was defective. Osiaa13 mutants also exhibited altered GUS staining controlled by the DR5 promoter in lateral root initiation sites. Furthermore, expression levels of several genes that might be associated with lateral root initiation were altered in Osiaa13. OsIAA13 encodes a member of the rice Aux/IAA protein family and is believed to play an essential part in blocking auxin signaling by repressing ARF function as a transcriptional regulator. The gain-of-function mutant OsIAA13 shares high sequence similarity with OsIAA11 and OsIAA30 in rice, and with AXR2/IAA7, SLR/IAA14, IAA16, and AXR3/IAA17 in Arabidopsis. Osiaa11 showed a similar phenotype to Osiaa13: the lateral root number of Osiaa11 was reduced because of a defect in LRP initiation but the crown root number was not reduced. Examination of the RiceXPro database revealed that OsIAA13, OsIAA11, and OsIAA30 show similar gene expression patterns indicating that the function of OsIAA30 might be redundant with OsIAA13 and OsIAA11 in lateral root initiation. OsIAA13, OsIAA11, and OsIAA30 may play an important role in lateral root formation but not in crown root formation.
OsIAA13 (Os03g0742900) sequence
Position: chr03:31291676..31294263 (- strand)
Length: 2588 bp
GGCTCCTCTC TCTTCTTCCA ACACCTCCTA ATCACCACAA AGGCTTAAAA ATCTCCAGTC TCCATAGAGA GAAAGAGAGA GAAGAGAAGG CAGCTTGATT TGGGGCGAGG AGACGCGATA GCCGCGATAG GCTAAGCAGC TGGCAGCGGC GGCTGCTGCG ATGGCCGGCG CCGACGTGGA CGTCGGGACG GAGCTCCGGC TGGGGCTGCC CGGAGGTGGC GGCGGCGCCG CCGAGGCGGC GGCCAAGGCC GCGAAGAGGG GCTTCGAGGA GACCATTGAC CTGAAGCTGA AGCTGCCCAC CGCCGGCATG GAGGAGGCCG CCGCCGGCAA GGCGGAGGCG CCGGCCGCCG AGAAGGCCAA GAGGCCGGCG GAGGCCGCCG CCGCCGACGC CGAGAAGCCA CCTGCTCCCA AGTATGTACT GGACCTAGCA TGATTTTATT ATTTTTTCAT ATATAAATCT GGCATTAATA TGTTACAGTA GTTCCAATTT GATCCTTCGT ATATATAGTT GCATCGTCTA ATTACTAGTT TACTTAATAA TTAGTGAGAA TTTAGTGGGA TTAGTATTGT TAATTTTTGC TAAAGGGATA AAAGATGAAT TATTTGGTGT GCTGTTTGGA GGAACTTTAT GCATGTTTAG TTTTGATTTA TGGAAAAAGA TCCACATATA GTAGCAGCAT AATGGCATTT GGTGAAAAGG TGTATGCATG TTTAGGTTAT TAGTTAGTTC TCCCCCCCCC CCCCCCCCCC TCTTCTCTTC CTTTCTTTTG TTTGGATTTA TATATTTGGG AGCTTGCTGC ATAAACTTGA GATGCCCATG AAGCAACACC TTTAAAAGAA GCACAAATTA TCCATATAAA AGAGATCCTC TGGCAGAAAG TGCATATGGC GACACCATCG TGTATGTTCT TTGTCGCTTG CAAAGTGCTG AGCAGATAAT TATCACCATT TTTAATCACC ATTGGCTCAT CTGATGCCTG CTTTGTGCAG CAGGTGTCAG AAATTGCTGG CGATGTAGTG GTACTACTAT TGTTTTTGGT TTATTTTTTC CTTCATCTCC TGGCAGATTC CATCTGAAAA AGGAGTTGTT TTCGTATTGT TTTGATTTAT TTTTTTTCCT GAAAAGAAGA TAGGATCACA ACAAAATATT TAGAACGATC AGAAAAGGAG TACATCTGTA TACAATTAAT TCCTGAAATA ATCATGGTGG CCATGATTCA TGTGGCAATA ACTAAAGTTG ATGGCATTTT CATTATACAC ACAGTACTAC CATAAAGTTG CTGCAGGCAT ACACACATAA TACATTAGTT AACACAGAGT TGGTCGCCAG CAAGAATATT GTAACATGCA TGATCTAGCT GGATGGAACT TCCAGTCACG AAAGTAAATC ATCTCAGTTT TTTCACAGCG CCAATAAGTT AGAACAAGAT GTGAAAAAAC TGAGCAAAAG AAAAAAAAAT CGATGTAGTA GCTAATTTCC AATGTTACAC ATCAGGGCGC AGGCAGTGGG TTGGCCACCA GTTCGGTCGT TCCGCAGGAA CATCATGACC GTCCAGTCAG TGAAGAGCAA GAAGGAAGAG GAAGCTGACA AGCAGCAGCA GCAGCCCGCT GCCAATGCCA GCGGCAGCAA CAGCTCTGCC TTCGTGAAGG TCAGCATGGA CGGGGCACCC TACCTGCGCA AGGTGGACCT GAAGATGTAC AACAGCTACA AGGACCTCTC CCTTGCTCTG CAGAAGATGT TCGGCACCTT CACCGCAACT GGTAAATCGC TGGCCACGGG AACCTGCAGA AACAGATTAT CAACTAGTAA TTAATTTGAA TGCAAAGTTT GATCCGGAAT TGATGAAAAC ACTGTGCATG AACCTCTCTG TCAGGGAACA ATATGAATGA GGTGAATGGC TCTGATGCTG TTACAACTTA TGAAGACAAG GATGGTGACT GGATGCTTGT TGGAGATGTC CCGTGGCAGT ATGTTACTAC TTCATTCTTG TGCCCCCTTA ACTGTTTTCC TTATTTTCCA CCTCATGATT AGAATGCGGT TTAGCGATAC CACGTTCAAG TTTCTCGCAT TAGCTGATTG GTTACTCTGC AGGATGTTTG TCGAGTCATG CAAACGCTTG AGGATCATGA AGGGTTCCGA AGCCATTGGT CTTGGTCAGT ATCTTCAGCT AAAATTTGCT TTCGTGTCAA GATTTATATC TTAATTCACA CCCCTTGTGT GTGTGGTTTT TCTTCCAGCA CCAAGAGCAA AGGACAAGTA CAAGAACAAG AGCTGAAAAT GTTGCAGTAT TAAGATGCTG AGCATGGTGC CATAAGAATG GTGTTCTTGC AGTGTGTTAT GGTTTCCTTA GAAATATAAG GTTTTACTTG TTTAGCTGGA TTAAAAGAAG GAAGAGTTGT CATGTTTAAT TTGTTTATGG ACCAGAAGCA ATAATCTATT TGTGTGTAGC TGGATTGGTG TTATGTGATG TCCCCTACCG TGTGTCCCAA GATCTACTTG ATGATTTTAT TCCAATGTTG TTAATTTAAG TTTGTGTGAT TGTGAAGTAA AGGGTATCAA TGAGACTATT ATTATTATGG TATCTGGT
Yuka Kitomi;Hiroki Inahashi;Hinako Takehisa;Yutaka Sato;Yoshiaki Inukai, OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice, Plant Science, 2012, 190: 116-122
M. Jain, N. Kaur, R. Garg, J.K. Thakur, A.K. Tyagi, J.P. Khurana, Structure expression analysis of early auxin-responsive Aux/IAA gene family in rice (Oryza sativa), Functional and Integrative Genomics 6 (2006) 47–59.