Os12g0612700
The rice Os12g0612700 was reported as OsHox33 in 2013 [1] by researchers from China.
Contents
Annotated Information
Function
- OsHox33 plays an important role in age-dependent leaf senescence in rice.
- OsHox33 is a member of the class III homeodomain-leucine zipper gene family which can accelerate leaf senescence in rice.
- OsHox33 can regulate the expression of GS1 and GS2, two senescence-associated genes.
Phenotypic analysis
- To reveal the function of OsHox33 in rice, the researchers constructed two RNAi vectors using specific fragments from the 3′-UTR or 5′-end region (5′-UTR and first exon) of OsHox33. These two RNAi constructs and two empty vectors were introduced into wild-type plants to produce transgenic plants.
- The researchers obtained 47 T0 transgenic plants of 16 independent lines with the RNAi 3′-UTR construct, and 61 transgenic plants of 23 independent lines with the RNAi 5′-end construct.
- The researchers randomly selected 15 lines with the 5′-end RNAi construct and 10 lines with the 3′-UTR RNAi construct to obtain T1 generation plants. Four of 15 lines with the 5′-end RNAi construct exhibited leaf senescence phenotypes. These plants initially displayed lesion spots in and around leaf midribs; the spots eventually expanded and increased throughout all parts of the leaves, leading to leaf wilting and death (Figure 2D and E). Two of 10 3′-UTR RNAi construct lines exhibited a similar but more severe phenotype: in addition to the appearance of lesion spots, yellowing occurred at leaf tips and spread to the rest of the leaf, leading to leaf wilting and death (Figure 2A–C).
- In contrast, two empty transgenic lines exhibited a normal phenotype, indicating that knockdown of OsHox33 accelerated rice leaf senescence.
Expression
- OsHox33 is mainly expressed in young organs and actively dividing cells, and may play crucial roles in rice growth and development
You can also add sub-section(s) at will.
Labs working on this gene
- College of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, China;
- Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
- School of Life Science, Tsinghua University, Beijing 100084, China;
- Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China