Os12g0612700

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The rice Os12g0612700 was reported as OsHox33 in 2013 [1] by researchers from China.

Annotated Information

Function

  • OsHox33 plays an important role in age-dependent leaf senescence in rice.
  • OsHox33 is a member of the class III homeodomain-leucine zipper gene family which can accelerate leaf senescence in rice.
  • OsHox33 can regulate the expression of GS1 and GS2, two senescence-associated genes.

Phenotypic analysis

  • To reveal the function of OsHox33 in rice, the researchers constructed two RNAi vectors using specific fragments from the 3′-UTR or 5′-end region (5′-UTR and first exon) of OsHox33. These two RNAi constructs and two empty vectors were introduced into wild-type plants to produce transgenic plants.
  • The researchers obtained 47 T0 transgenic plants of 16 independent lines with the RNAi 3′-UTR construct, and 61 transgenic plants of 23 independent lines with the RNAi 5′-end construct.
  • The researchers randomly selected 15 lines with the 5′-end RNAi construct and 10 lines with the 3′-UTR RNAi construct to obtain T1 generation plants. Four of 15 lines with the 5′-end RNAi construct exhibited leaf senescence phenotypes. These plants initially displayed lesion spots in and around leaf midribs; the spots eventually expanded and increased throughout all parts of the leaves, leading to leaf wilting and death (Figure 2D and E). Two of 10 3′-UTR RNAi construct lines exhibited a similar but more severe phenotype: in addition to the appearance of lesion spots, yellowing occurred at leaf tips and spread to the rest of the leaf, leading to leaf wilting and death (Figure 2A–C).
  • In contrast, two empty transgenic lines exhibited a normal phenotype, indicating that knockdown of OsHox33 accelerated rice leaf senescence.


Figure 2. Phenotypes and expression analysis of OsHox33 RNAi lines.[1].

Expression

  • OsHox33 is mainly expressed in young organs and actively dividing cells, and may play crucial roles in rice growth and development

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Labs working on this gene

  • College of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, China;
  • Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
  • School of Life Science, Tsinghua University, Beijing 100084, China;
  • Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China

References

  1. 1.0 1.1 Luan W, Shen A, Jin Z, Song S, Li Z, Sha A. Knockdown of OsHox33, a member of the class III homeodomain-leucine zipper gene family, accelerates leaf senescence in rice. Sci China Life Sci. 2013 Dec;56(12):1113-23. doi: 10.1007/s11427-013-4565-2. Epub 2013 Dec 5. PubMed PMID: 24302292.

Structured Information