Os12g0506800

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The rice gene Os12g0506800 was reported as OsAtg10b in 2009[1] by researchers from Korea and Japan.

Annotated Information

Gene Symbol

  • Os12g0506800 <=> OsATG10b, Atg10b

Function

  • OsATG10b plays an important role in the survival of rice cells against oxidative stresses.

Mutation

Fig. 6. Effect of high salt or methyl viologen on WT and mutants. [1].
  • The researchers identified T-DNA insertional mutants in that gene. Those osatg10b mutants were sensitive to treatments with high salt and methyl viologen (MV). Monodansylcadaverine-staining experiments showed that the number of autophagosomes was significantly decreased in the mutants compared with the WT. Furthermore, the amount of oxidized proteins increased in MV-treated mutant seedlings.

Expression

  • GUS assay indicated that OsAtg10b was highly expressed in the mesophyll cells and vascular tissue of younger leaves, but its level of expression decreased in older leaves.

Sublocalization

  • To locate it within a cell, OsATG10b protein was fused to the Nterminal region of sGFP and introduced into protoplasts isolated from Oc cells. We found that this fusion protein was localized in the cytosol (Fig. 3A), which was merged with that of mRFP (Figs. 3B and 3C).
Fig. 3. Sub-cellular localization of OsATG10b::sGFP fusion protein. [1].

Evolution

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Labs working on this gene

  • National Research Laboratory of Plant Functional Genomics, POSTECH Biotech Center, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea,
  • Plant Science Center, The Institute of Physical and Chemical Research, Yokohama 230-0045, Japan,
  • Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan,
  • Graduate School of Biotechnology and Plant Metabolism

Research Center, Kyung Hee University, Yongin 449-701, Korea

References

  1. 1.0 1.1 1.2 Shin JH, Yoshimoto K, Ohsumi Y, Jeon JS, An G. OsATG10b, an autophagosome component, is needed for cell survival against oxidative stresses in rice. Mol Cells. 2009 Jan 31;27(1):67-74. doi: 10.1007/s10059-009-0006-2. PubMed PMID: 19214435.


Structured Information