Os09g0457600
The rice Os09g0457600 was reported as Amy3B in 2012 [1] by researchers from Japan.
Contents
Annotated Information
Figure 1. a-Amylase activity and expression levels of eight a-amylase genes during grain filling.[1].
Gene Symbol
- Os09g0457600 <=> OsAmy3B,Amy3B
Function
- Amy3B is a member of α-Amylase (EC3.2.1.1) gene family, which encodes a starch-hydrolyzing enzyme.
- Activation of a-amylase by high temperature is a crucial trigger for grain chalkiness and that its suppression is a potential strategy for ameliorating grain damage from global warming
- α-amylase is one of the enzymes involved in the first committed step for degradation of reserve starch. During the process, synthesis of a-amylase is up-regulated by a plant hormone, gibberellin (GA), but down-regulated by another hormone, abscisic acid (ABA), and by sugars.
Expression
- A series of quantitative real-time RT-PCR analyses using gene-specific primer sets for respective a-amylase genes revealed that the expression of Amy1C and Amy3A as well as Amy1A, Amy3D and Amy3E was up-regulated under high temperature (33 °C/28 °C) in the middle of the ripening stage at around 15 days after flowering (DAF; Figure 1b).
- Amy1A and Amy1C showed a similar pattern of expression in the high temperature plot, gradually increasing from 8 DAF and peaking at 15–20 DAF. Amy3A and Amy3D expression peaked around 15 DAF, while Amy3E showed high levels at 8–30 DAF in the high temperature plot. In contrast, the expression of Amy2A, Amy3B and Amy3C was not induced by high temperature (Figure 1b).
- The intensity of the expression of all a-amylase genes in the course of grain filling clearly indicates that the newly characterized Amy3A gene was expressed at a considerable level under high temperature from 15 DA.
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Labs working on this gene
- National Agricultural Research Center, Joetsu, Japan
- RIKEN Plant Science Center, Tsurumi, Yokohama, Japan
- Niigata University, Ikarashi, Niigata, Japan
References
