Os09g0416800

The rice Os09g0416800 was reported as OsCAF1G in 2014 by researchers from China.

Annotated Information

Figure 6. Subcellular localization of OsCAF1–GFP fusion proteins in onion epidermal cells..

Gene Symbol

• Os09g0416800 <=> OsCAF1G,CAF1G

Function

• CAF1 belongs to the RNase D group in the DEDD superfamily, and is a protein conserved through evolution from yeast to humans and plants.
• Researchers identified and cloned four OsCAF1 genes (OsCAF1A, OsCAF1B, OsCAF1G, and OsCAF1H) from rice. Point mutations in the catalytic residues of each analyzed recombinant OsCAF1 proteins were shown to disrupt deadenylase activity.
• OsCAF1 proteins may be involved in the deadenylation in rice.

Expression

• The unique expression patterns of each OsCAF1 were observed in various tissues when undergoing abiotic stress treatments, implying that each CAF1 gene in rice plays a specific role in the development and stress response of a plant.

Subcellular localizations

• To determine the subcellular localizations of OsCAF1 proteins, OsCAF1 genes were fused with GFP under the control of a 35S promoter and introduced into onion epidermal cells using particle bombardment.
• As shown in Fig. 6b, fluorescence signals from GFP fused to the C terminal of each OsCAF1 protein (OsCAF1–GFP) were present not only in the nucleus but also in the cytoplasm, the same cellular localization pattern as GFP only.
• In the cytoplasm, OsCAF1A–, OsCAF1G– and OsCAF1H–GFP protein signals were distributed uniformly (Fig. 6b), whereas OsCAF1B (L)–GFP was localized to specific cytosolic foci and formed filamentous structures (Figs. 6b, c).
• Similar subcellular localization patterns were observed when GFP fused to the N-terminus of each OsCAF1 (GFP–OsCAF1). We further examined the subcellular localization of OsCAF1 proteins by fusing another reporter, β-glucuronidase (GUS), in onion epidermal cells.
• Each OsCAF1 fusion proteins (OsCAF1–GUS) were distributed both in the nucleus and cytoplasm, whereas GUS was predominantly localized in the cytoplasm. The OsCAF1B–GUS was also localized to specific cytosolic foci and filamentous structures.

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Labs working on this gene

• Department of Life Sciences, National Central University, Jhongli City, Taoyuan County 320, Taiwan, ROC
• Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Jhongli City, Taoyuan County 320, Taiwan, ROC
• Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan, ROC