Os08g0531600

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Os08g0531600(OsSPL16) is a very important gene that encoding a positive regulator of cell prolifertion which can pormote cell division and grain filling.

Annotated Information

qGW8 within a genetic window on the long arm of chromosome 8 defined by the markers RM80 and RM447.The progeny testing of homozygous recombinant plants allowed this region to be narrowed to an ~7.5-kb stretch flanked by RM502 and PSM711.Quantitative trait locus GW8 is synonymous with OsSPL16, which encodes a protein that is a positive regulator of cell proliferation.Higher expression of this gene promotes cell division and grain filling, with positive consequences for grain width and yield in rice. Conversely, a loss-of-function mutation in Basmati rice is associated with the formation of a more slender grain and better quality of appearance. The correlation between grain size and allelic variation at the GW8 locus suggests that mutations within the promoter region were likely selected in rice breeding programs. We also show that a marker-assisted strategy targeted at elite alleles of GS3 and OsSPL16 underlying grain size and shape can be effectively used to simultaneously improve grain quality and yield.

Function

Figure1 Parental grains. Scale bar, 3 mm.

OsSPL16 control of grain size, shape, and quality[1]. OsSPL16 encodes a protein that is a positive regulator of cell proliferation. OsSPL16contributes to organ size through its effect on the cell cycle machinery.Higher expression of this gene promotes cell division and grain filling, with positive consequences for grain width and yield in rice. The candidate gene 'OsSPL16' encodes squamosa promoter-binding protein-like 16, which belongs to the SBP domain family of transcription factors and shares homology with the product of tga1,a domestication syndrome gene associated with the formation of grains in maize[1]. Grain width and length were also altered in NIL-qw8 plaints expressing the Basmati385 OsSPL16 cDNA under the control of the native HJX74 promoter.The SPL genes have an important role in the control of flowering.The SPL genes have been shown to be regulated by microRNA miR156 (refs. 14–16,19–22), and OsSPL16contains an OsmiR156 target sequence. OsSPL16functions as a negative regulator of panicle branching.

Mutation

Higher expression of this gene promotes cell division and grain filling, with positive consequences for grain width and yield in rice.A loss-of-function mutation in Basmati rice is assciated with the formation of a more slender grain and better quality of apperance. The correlation between grain size and allelic variation at the GW8 locus suggests that mutations within the promoter region were likely selected in rice breeding programs[1].

Expression

Figure2 Expression of OsSPL16in NIL-GW8and NIL-gw8.R, root; C, culm; L, leaf blade; SAM, shoot apex meristem; BM, branch meristem; YP1–Y22, young panicles, where the number indicates the length of the panicle in centimeters. Expression levels are shown as relative number of copies per 1,000 copies of rice actin3. Data are given as mean ±s.e.m. (n= 4).

The expression profiles of OsSPL16in various organs of HJX74 were examined by quantitative RT-PCR analysis. OsSPL16 was preferentially expressed in developing panicles, and the highest levels of OsSPL16expression were found in panicles of 7 cm in length, whereas there was less transcript accumulation in the root, culm, leaf sheath, shoot meristem and young panicle(of <1 cm in length)[1]

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Labs working on this gene

The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, National Centre for Plant Gene Research, Beijing, China. The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China. The State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. working Development of the SSSL population.Fine mapping of qGW8.Transgene constructs.Expression analysis.Histological analysis.Transactivation activity assay.Analysis of transgenic plants expressing OsSPL16.

References

  1. 1.0 1.1 1.2 1.3 Wang S, Wu K, Yuan Q, et al. Control of grain size, shape and quality by OsSPL16 in rice[J]. Nature genetics, 2012, 44(8): 950-954.

Structured Information