Os07g0592200

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The rice Os07g0592200 was reported as OsAP65 in 2013 [1] by researchers from China.

Annotated Information

Figure 2. Pollen grains from OsAP65 T-DNA insertional mutant and wild-type plants.[1].

Gene Symbol

  • Os07g0592200 <=> OsAP65,AP65

Function

  • OsAP65 encodes a rice aspartic protease
  • OsAP65 is essential for male fertility and plays a role in pollen germination and pollen tube growth.

Phenotypic analysis

  • The result showed that the total number of pollen grains in an anther and the percentage of mature pollen of OsAP65+/– plants were not different from those of OsAP65+/+ plants.
  • DAPI staining showed that all pollen grains from both OsAP65+/– and OsAP65+/+ plants contained three nuclei in the mature pollen: two bright, intensely stained sperm nuclei and one diffuse, weakly stained vegetative nucleus (Fig. 2A, B).
  • This analysis indicated that the mutation did not affect sperm cell development and division. SEM was used to examine the surface of the pollen grains, and no significant difference in pollen morphology could be detected between the OsAP65+/– and OsAP65+/+ plants (Fig. 2C, D, F, G).
  • TEM scanning of pollen grains at maturity did not reveal subcellular changes between the OsAP65+/– and OsAP65+/+ plants (Fig. 2E, H). This also implies that disruption of OsAP65 does not cause a visible difference in pollen morphology.
  • Pollen germination and pollen tube elongation were then examined in vitro and in vivo. The percentage of germinated pollen grains in vitro was found to be significantly lower in OsAP65+/– plants (56.78%) than in OsAP65+/+ plants (79.64%) (Fig. 2I, J, L).

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Labs working on this gene

  • National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China

References

  1. 1.0 1.1 Huang J, Zhao X, Cheng K, Jiang Y, Ouyang Y, Xu C, Li X, Xiao J, Zhang Q. OsAP65, a rice aspartic protease, is essential for male fertility and plays a role in pollen germination and pollen tube growth. J Exp Bot. 2013 Aug;64(11):3351-60. doi: 10.1093/jxb/ert173. PubMed PMID: 23918968; PubMed Central PMCID: PMC3733154.

Structured Information