Os07g0409900
The rice Os07g0409900 was reported as CPK18 in 2014 [1] by researchers from China.
Contents
Annotated Information
Figure 7. A Working Model for the CPK18-MPK5 Pathway.[1].
Gene Symbol
- Os07g0409900 <=> CPK18,OsCPK18
Function
- OsCPK18 is a rice (Oryza sativa) calcium-dependent protein kinase (CDPK), it is as an upstream kinase of MAPK (MPK5) in vitro and in vivo.
- OsCPK18 was shown to phosphorylate and activate MPK5 without affecting the phosphorylation of its TXY motif. Instead, CPK18 was found to predominantly phosphorylate two Thr residues (Thr-14 and Thr-32) that are widely conserved in MAPKs from land plants.
- Further analyses reveal that the newly identified CPK18-MPK5 pathway represses defense gene expression and negatively regulates rice blast resistance.
CPK18-MPK5 Pathway Model
- Plasma membrane–associated CPK18 is regulated by Ca 2+ signals to activate MPK5 through Thr-14/Thr-32 phosphorylation (pT14/pT32). Activated MPK5 then phosphorylates different transcription factors to activate or repress downstream gene expression.
- The CPK18-MPK5 pathway may work independently or cooperatively with the canonical MAPK cascade, which is activated by pathogen-associated molecular pattern (PAMP) and pattern recognition receptor (PRR) interaction.
- MPK5 likely exists in multiple phosphorylated forms with variable activities. Arrows indicate positive regulation; the blunt-ended line indicates negative regulation. Dashed arrows indicate undefined pathways of MPK5 feedback phosphorylation of CPK18.
Subcellular Localization
- Transient expression of green fluorescent protein (GFP)–tagged MPK5 and CPK18 in onion (Allium cepa) epidermal cells and rice protoplasts showed that MPK5 was localized in the nucleus and cytoplasm, whereas CPK18 was predominantly associated with the plasma membrane.
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Labs working on this gene
- Department of Plant Pathology and Environmental Microbiology, The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802
