Os07g0209000
The rice Os07g0209000 was reported as OsDGL1 in 2012 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os07g0209000 <=> OsDGL1,DGL1
Function
- The OsDGL1 encodes the dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48 kDa subunit precursor.
- OsDGL1 had a similar function in rice to that of its homolog AtDGL1 in Arabidopsis.
- OsDGL1 is an essential protein subunit of the oligosaccharyl-transferase (OST) complex, which is involved in N-glycosy-lation in eukaryotes.
Phenotypic analysis
- The roots of the Osdgl1 mutant were more fragile than those of the WT. Staining of 7-day-old seedling roots with Evans blue (Gaff and Okong’O-gola 1971) revealed large patches of stained cells along the roots of Osdgl1 mutant plants (Fig. 2a–d), especially near the root tips, indicating that cell death had occurred.
- Basal H2O2 concentrations were higher in the roots of the Osdgl1 mutant plants compared with WT seedlings (Fig. 2i–l). H2O2 accumulated at the root tip where cell death was observed, as evidenced by Evans blue staining (Fig. 2a–d). The degree of NBT staining, and hence the amount of O2-in the roots, was similar in WT and Osdgl1 (Fig. 2e–h).
- The leaky rice mutant, Osdgl1, displayed a change of matrix polysaccharides in its root cell wall, shorter root cell length, smaller root meristem and cell death in the root. Consistent with the known function of the OST complex in eukaryotes, the Osdgl1 mutation leads to a defect in N-glycosylation in the root.
Evolution
- OsDGL1 is an ortholog of Arabidopsis DGL1, human OST48 and yeast WBP1.
You can also add sub-section(s) at will.
Labs working on this gene
- The State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, PR China
- Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, PR China