Os06g0354700
The rice gene Os06g0354700 was reported as nyc3 in 2009[1] by researchers from Japan.
Contents
Annotated Information
Gene Symbol
- Os06g0354700 <=> nyc3, osnyc3
Function
- NYC3 encodes a plastid-localizing a/b hydrolase-fold family protein with an esterase/lipase motif.
Mutation
Figure 1. The stay-green phenotype of nyc3. [1].
- nyc3 retained a higher chlorophyll a and chlorophyll b content than the wild-type but showed a decrease in other senescence parameters during dark incubation, suggesting that it is a non-functional stay-green mutant. In addition, a small amount of pheophytin a, a chlorophyll a-derivative without Mg2+ ions in its tetrapyrrole ring, accumulated in the senescent leaves of nyc3. nyc3 shows a similar but weaker phenotype to stay green (sgr), another non-functional stay-green mutant in rice. The chlorophyll content of nyc3 sgr double mutants at the late stage of leaf senescence was also similar to that of sgr.
Expression
Figure 7. The RT-PCR analysis of NYC3 and SGR expression. [1].
- The RT-PCR analysis revealed that NYC3 is expressed at a low level in non-senescent leaves and at a high level in darkincubated leaves; however, the degree of induction is lower than that of SGR (Figure 7a). NYC3 is also expressed in non-photosynthetic tissues such as the roots and calli (Figure 7b). This is consistent with the observation that the growth of nyc3 homozygous calli was not sufficiently vigorous for transformation. Interestingly, it has also been reported that RCCR, one of the enzymes involved in chlorophyll degradation, is expressed in non-photosynthetic tissues and plays a role in programmed cell death [2]. NYC3 may therefore also play a role in non-photosynthetic tissues, at least in the callus.
Evolution
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Labs working on this gene
- Institute of Radiation Breeding, National Institute of Agrobiological Sciences, Hitachi-ohmiya 219–2293, Japan,
- Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, 2–1–2 Kannondai, Tsukuba, Ibaraki 305–860, Japan
- Graduate School of Science, Hiroshima University, Higashi-hiroshima 739–8526, Japan
References
- ↑ 1.0 1.1 1.2 Morita R, Sato Y, Masuda Y, Nishimura M, Kusaba M. Defect in non-yellow coloring 3, an alpha/beta hydrolase-fold family protein, causes a stay-green phenotype during leaf senescence in rice. Plant J. 2009 Sep;59(6):940-52. doi: 10.1111/j.1365-313X.2009.03919.x. Erratum in: Plant J. 2009 Dec;60(6):1110. PubMed PMID: 19453447.
- ↑ Yao, N. and Greenberg, J.T. (2006) Arabidopsis ACCELERATED CELL DEATH2 modulates programmed cell death. Plant Cell, 18, 397–411.
