Os05g0343400

The rice Os05g0343400 was reported as OsWRKY53 in 2007 by researchers from Japan.

Annotated Infomation

Gene Symbol

• Os05g0343400 <=> OsWRKY53,WRKY53

Function

• WRKY proteins form a large family of plant-specific transcription factors that specifically bind to the W-box elements (T)TGAC(C/T) and appear to play a regulatory role in a variety of stress responses.
• WRKY proteins have been investigated extensively for their possible involvement in defense responses against attacks by pathogens, mainly in dicotyledonous plants such as Arabidopsis and tobacco.
• The rice genome is predicted to contain over 100 WRKY (OsWRKY)genes.
• OsWRKY53 is a common transcription factor in the signal transduction pathways triggered by both chitin oligosaccharide and fungal cerebroside elicitors.
• OsWRKY53 is a transcription factor that plays important roles in elicitor-induced defense signaling pathways in rice.

Phenotypic analysis

• Overexpression of OsWRKY53 enhances resistance to rice blast fungus

Expression

• Northern hybridization revealed a significant increase in the level of WRKY53 mRNA at 6 h after inoculation, after which it decreased gradually

Up-regulated defense-related genes in OsWRKY53-overexpressing transgenic rice cells

Accession no.	Annotation	Fold change a	Chitin oligosaccharide elicitor response b
AK071613	PR-10 (PBZ1)	6.92	+
AK058896	PR-14	6.06	+
AK058921	PR-14	5.98	−
AK073267	chitinase	5.30	+
AK073978	peroxidase	4.18	−
AK111104	PR -5	3.97	+
AK102505	chitinase	3.67	+
AK102307	peroxidase	3.56	+
AK063935	cinnamoyl CoA reductase	2.74	−

Subcellular localization

• Os05g0343400 localizes to the nucleus and activates the expression of defense-related genes.

Evolution

figure 2.

figure 2.(continued)

WRKY proteins can possess one or more WRKY domains and, as with other transcription factors, the nature of the DNA-binding domain forms a basis for categorizing different members of the WRKY family. WRKY proteins containing a single WRKY domain are group II proteins; those with two are group I. The group II WRKY proteins are further subdivided intosubgroups a–e based on the presence of short conserved structural motifs . A third group, group III, contains a single WRKY domain with a variant zinc-finger CX7CX23HXC. Compared with domains from groups Ia and II proteins, the terminal histidine of the zinc-finger of groups Ib and III is replaced with cysteine and the spacing is altered within group III. Group IV WRKY proteins contain the WRKY motif, but lack a complete zinc-finger.

numerous WRKY gene duplications occurred after the divergence of the monocotyledons from dicotyledons some 50–80 million years ago. Early origin for the WRKY family in primitive eukaryotes, before the emergence of the plant phyla, and its gross expansion during the course of plant evolutionary radiation, likely because of selective pressures favoring greater adaptability. Group I WRKY genes represent the ancestral form, with other groups arising later through losses and gains of a WRKY domain, and that this family originated some 1.8–2 billion years ago. the C-terminal domains are required for DNA-binding activity, thus are constrained in their ability to mutate without losing function, whereas the N-terminal domains mediate protein-protein interactions and may be less functionally constrained . group Ia genes arose from a fusion of two group II WRKY genes evolved at an early time.

Labs working on this gene

• Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
• National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan
• Department of Life Science, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 216-0011, Japan
• Plant Acquired Immunity Research Unit, Discovery Research Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
• Meiji Seika Kaisha Ltd., 760 Morooka, Kohoku-ku, Yokohama, Kanagawa 222-8567, Japan