Os05g0154600
The rice Os05g0154600 was reported as APIP6 in 2012 [1] by researchers from China.
Contents
Annotated Information
Figure 2. Response of AvrPiz-t Transgenic Rice to Blast Infection, flg22, and Chitin Treatments.[1].
Function
- APIP6 in is a Functional RING Finger E3 Ubiquitin Ligase
- AvrPiz-t suppresses the ubiquitin ligase activity of the rice RING E3 ubiquitin ligase APIP6 and that, in return, APIP6 ubiquitinates AvrPiz-t in vitro.
- Agroinfection assays reveal that AvrPiz-t and AvrPiz-t Interacting Protein 6 (APIP6) are both degraded when coexpressed in Nicotiana benthamiana.
- Silencing of APIP6 in transgenic rice leads to a significant reduction of flg22-induced ROS generation, suppression of defense-related gene expression, and enhanced susceptibility of rice plants to M. oryzae.
Expression
- In both the T1 (primary transformants) and T2 generations, the RNAi plants grew normally and did not show any changes in growth and development.
- Blast inoculations showed that the APIP6 RNAi plants were as resistant as the wild-type Piz-t plants (TRD) to the isolate, suggesting that APIP6 is not required for the Piz-t-mediated resistance to the avirulent strain.
- Punch inoculation assays showed that lesions were larger on the APIP6 RNAi plants than on the segregated wild-type plants (Figure 7A).
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Labs working on this gene
- Department of Plant Pathology, Ohio State University, Columbus, OH 43210
- State Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
- Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
- State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021 China
- Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506
- Department of Horticulture and Crop Science, Ohio State University, Columbus, OH 43210
References
