Os04g0433800

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The rice Os04g0433800 was reported as OsRecQl4 in 2013 [1] by researchers from Japan.

Annotated Information

Figure 1B. Northern blot analysis.[1].

Gene Symbol

  • Os04g0433800 <=> OsRecQl4,RecQl4,OsMed34_1,Med34_1

Function

  • OsRecQl4 plays an important role as a caretaker, maintaining genome stability during DNA replication stress in the rice meristem.

Phenotypic analysis

  • Osrecql4 mutant plants showed hypersensitivity to DNA damaging agents and enhanced frequency of HR compared to wild-type (WT) plants.

Expression

  • Expression of OsRecQl4 was analyzed by northern blot in 7-day-old seedlings of the japonica-type rice cultivars Dongjin or Nipponbare. Using probe A (Figure 1A), relatively strong signals were observed in the basal parts of shoots (BS, as defined in Figure 1B) including the shoot apical meristem (SAM), and weak signals were observed in the root (R). No signal was detected in the lower (LS) or upper (US) shoot (Figure 1B).
  • OsRecQl4 was highly expressed in the RAM expression of OsRecQl4 was restricted to meristematic tissues.

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Labs working on this gene

  • Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
  • Functional Plant Research Unit, National Institute of Agrobiological Sciences,2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
  • Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Sakura, Saitama 338-8570, Japan.
  • Graduate School of Nanobioscience, Yokohama City University, 22-2, Seto, Kanazawa, Yokohama 236-0027, Japan.
  • Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka, Yokohama 244-0813, Japan.

References

  1. 1.0 1.1 Kwon YI, Abe K, Endo M, Osakabe K, Ohtsuki N, Nishizawa-Yokoi A, Tagiri A, Saika H, Toki S. DNA replication arrest leads to enhanced homologous recombination and cell death in meristems of rice OsRecQl4 mutants. BMC Plant Biol. 2013 Apr 12;13:62. doi: 10.1186/1471-2229-13-62. PubMed PMID: 23586618; PubMed Central PMCID: PMC3648487.

Structured Information