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OsCPK10 is a member of OsCPKs family including 29 CPKs in rice. Calcium-dependent protein kinases (CDPKs) are improtant calcium sensors icluded in plant responses to pathogen infection. The expression of OsCPK10 was strongly induced following treatment with a Magnaporthe grisea elicitor. Kinase activity assay showed that the functional OsCPK10 protein not only autophosphorylated, but also phosphorylated Casein in a calcium-dependent manner. Overexpression of constitutively active OsCPK10 in Arabidopsis enhanced the resistance to infection with Pseudomonas syringae pv. tomato, associated with elevated expression of both SA- and JA-related defense genes. Similarly, transgenic rice plants containing constitutively active OsCPK10 exhibited enhanced resistance to blast fungus M. grisea. Collectively, rice OsCPK10 is a crucial regulator in plant immune responses, and its overexpression may regulate disease resistance by activating both SA- and JA-dependent defense responses.
OsCPK10 has a variable domain (residues 1-132), a kinase domain (residues 133-396), a junction domain (residues 397-427) and a Calmodulin-like domain (residues 428-599). Four EF-hands were found in the Calmodulin-like domain (residues 438-466, 474-502, 510-538, and 544-572).
The expression of OsCPK10, in M.grisea elicitor-treated rice calli, shows a rapid activation and peaks at 1 hour, which is earlier than that of OsPAL1 expression. This result indicates that OsCPK10 is an early pathogen-responsive gene.
Isolation and sequence analysis
OsCPK10 is isolated by PT-PCR from the total RNA extraction using specific primer pairs and the coding region of OsCPK10 is 1800 bp in length which encodes 599 amino acids. (Primers:forward 5'-ATGGGGAACACGTGCGTC-3' and reverse 5'-TCAAGACATCCTCAAGGCCTC-3'). The PCR products were ligated to a pEASY-T1 vector (TransGen Biotech, China).
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Labs working on this gene
State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China
 B. Wan, Y. Lin, T. Mou, Expression of rice Ca2+-dependent protein kinases (CDPKs) genes under different environmental stresses, FEBS Lett. 581 (2007)1179e1189  Fu L, Yu X, An C. Overexpression of constitutively active OsCPK10 increases Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea. Plant Physiol Biochem. 2013 Dec;73:202-10. doi: 10.1016/j.plaphy.2013.10.004. Epub 2013 Oct 11. PubMed PMID: 24141028.