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Os03g0755100,which also called OsALS1 and localized at the tonoplast, is responsible for sequestration of (aluminum) Al into the vacuoles, which is required for
internal detoxification of Al in rice. The expression of OsAIS1 is regulated by ART1, a C2H2-type zinc finger transcription factor. OsALS1 encodes a half-size ABC transporter that is a member of the TAP (transporter associated with antigen processing) sub-group.OsALS1is localized at the tonoplast and expressed in all tissues. Some investigations have demonstrated knockout of OsALS1 resulted in increased sensitivity to AI. Expression of OsALS1 was rapidly and specifically induced by Al in the roots, but not by other metals or low pH. OsALS1 (Os03g0755100) has 17 exons and 16 introns, and encodes a peptide with 641 amino acids. OsALS1 is a single-copy gene in the rice genome. Phylo-genetic analysis identified homologs of OsALS1 in various species, from moss (Physcomitrella patens) and fern( Selaginella moellendorffii ) to higher plants including monocots and dicots. The identity of OsALS1 to AtALS1 is 72% at the amino acid level. The closest homolog is AAM47580 from Sorghum bicolor, which shares 91% identity with OsALS1[1.2].
The green fluorescence of OsALS1–GFP was localized outside of the nucleus and did not overlap with the DsRed signal, which was localized at the nucleus and cytosol.Furthermore, subcellular localization of OsALS1 was also examined by introducing OsALS1–GFP to rice leaf proto-plasts. The signal was observed at the tonoplast.Taken together, these results indicate that OsALS1 is local-ized to the tonoplast.
The Os03g0755100 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, and rice.
Labs working on this gene
1 Department of Biochemistry, University of California, Boyce Hall, Riverside, CA 92521, USA 2 Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan
1 Chao-Feng Huang, Naoki Yamaji, Zhichang Chen and Jian Feng Ma. A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice.The Plant Journal (2012) 69, 857–867. 2 Paul B. Larsen, Jesse Cancel, Megan Rounds, Vanessa Ochoa.Arabidopsis ALS1 encodes a root tip and stele localized half type ABC transporter required for root growth in an aluminum toxic environment.Planta (2007) 225:1447–1458.