Os03g0226400

The rice Os03g0226400 was reported as MTP8.1 in 2013 by researchers from Japan.

Annotated Information

Figure 4. Subcellular localization of OsMTP8.1..

Gene Symbol

• Os03g0226400 <=> MTP8.1,OsMTP8.1

Function

• OsMTP8.1 encodes a cation diffusion facilitator (CDF) family member.
• The expression of OsMTP8.1 was found to enhance Mn accumulation and tolerance in S. cerevisiae.
• Disruption of OsMTP8.1 resulted in decreased chlorophyll levels, growth inhibition in the presence of high concentrations of Mn, and decreased accumulation of Mn in shoots and roots. However, there was no difference in the accumulation of other metals, including Zn, Cu, Fe, Mg, Ca, and K.
• OsMTP8.1 is an Mn-specific transporter that sequesters Mn into vacuoles in rice and is required for Mn tolerance in shoots.

Expression

• In plants, OsMTP8.1 and its transcript were mainly detected in shoots. High or low supply of Mn moderately induced an increase or decrease in the accumulation of OsMTP8.1, respectively.

Subcellular localization

• OsMTP8.1:GFP fusion protein was transiently expressed in onion epidermal cells. The fluorescence of cells transfected with GFP alone was detected in the nucleus and cytoplasm (Fig. 4A). In contrast, the fluorescence of the fusion protein was observed at the cell periphery (Fig. 4B) but not in the nucleus (magnified view in Fig. 4B), suggesting that OsMTP8.1 localized to the tonoplast.

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Labs working on this gene

• Faculty of Agriculture, Kochi University, Nankoku 783-8502 Japan
• Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan
• Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
• Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan