Os02g0178800
OsGL1-2 is a member of the GL1-like genes in rice[1].
Contents
Annotated Information
Function
- The full-length cDNA of OsGL1-2 was isolated from Minghui 63 by gene-specific primers and cloned into pCAMBIA1301S under the control of Cauliflower mosaic virus 35S promoter[1].
- OsGL1-2 gene has a significant role in controlling the wax layer and thus protecting leaves from water loss in rice[1].
GO assignment(s): GO:0003824,GO:0008152
Mutation
- Among 23 independent transgenic plants generated, nine transgenic plants showed high expression level of the transgene[1].
- Compared to the over-expression and wild type plants, the osgl1-2 mutant was more sensitive to drought stress at reproductive stage, suggesting an important role of this gene in drought resistance[1].
- The crystallization patterns of cuticular waxes on matured leaves of the osgl1-2 mutant were significantly different and reduced compared to OsGL1-2 overexpression and WT plants[1].
- The osgl1-2 mutant showed rapid water loss and decreased epidermal permeability compared to the OsGL1-2 over-expression and WT plants, suggesting that the mutant plants may be susceptible to drought[1].
Expression
- Compared to the wild type, the OsGL1-2-over-expression rice exhibited more wax crystallization and a thicker epicuticular layer; while the mutant of this gene showed less wax crystallization and a thinner cuticular layer[1].
- The expression levels of OsGL1-1 and OsGL1-2 were gradually increased throughout the time course, whereas the OsGL1-6 showed strong induction only at 2 day after treatment[1].
- OsGL1-2 was induced by ABA treatment, and the highest induction was at 12 h after the treatment. RT-PCR analysis showed that the transcript of OsGL1-2 gene was broken down in the 04Z11ML82 mutant[1].
- The OsGL1-2-overexpresion plants showed a significant decrease of chlorophyll leaching and the osgl1-2 mutant plants showed a significant increase of chlorophyll leaching, which indicates that over-expression of OsGL1-2 genes decreased the cuticular permeability and perhaps can cause drought tolerance of the plants[1].
Evolution
GL1 shows high sequence similarity with OsGL1-1(76%), OsGL1-2 (62%), and OsGL1-3 (62%), suggesting that the OsGL1-1~3 may be homologs of GL1[1].
Knowledge Extension
- The outermost surfaces of plants are covered with an epicuticular wax layer that provides a primary waterproof barrier and protection against different environmental stresses. Glossy 1 (GL1) is one of the reported genes controlling wax synthesis[1].
Labs working on this gene
- National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070 Wuhan, China
- Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh
