Os02g0148100
OsMAPK33 (Os02g0148100) is a Mitogen-activated protein kinases (MAPK) cDNA clone in rice, which is mainly induced by drought stress
Contents
Annotated Information
Function
Under dehydration conditions, the suppressed lines showed lower osmotic potential compared with that of wild-type plants, suggesting a role of OsMAPK33 in osmotic homeostasis. Nonetheless, the suppressed lines did not display any significant difference in drought tolerance compared with their wild-type plants.
With increased salinity, there was still no difference in salt tolerance between OsMAPK33-suppressed lines and their wild-type plants. However, the overexpressing lines showed greater reduction in biomass accumulation and higher sodium uptake into cells, resulting in a lower K+/Na+ ratio inside the cell than that in the wild-type plants and OsMAPK33-suppressed lines. These results suggest that OsMAPK33 could play a negative role in salt tolerance through unfavourable ion homeostasis. Gene expression profiling of OsMAPK33 transgenic lines through rice DNA chip analysis showed that OsMAPK33 altered expression of genes involved in ion transport.[1]
Expression
OsMAPK33 is found to exhibit organ-specific expression with relatively higher expression in leaves as compared with roots or stems, and to exist as a single copy in the rice genome.[1]
Evolution
OsMAPK33 showed approximately 47–93% identity at the amino acid level with other plant MAPKs.[1]
Sequence
analysis revealed that the cDNA, renamed as OsMAPK33 (Os02g0148100), had a high degree of similarity to the Ser/Thr protein kinase genes (MAPKs) of other plant species. OsMAPK33 contained 11 conserved subdomains and the phosphorylation activation motif (TEY) found in Ser/Thr protein kinases. OsMAPK33 was found to be the most closely related to OsMAP2 (92.7%) as previously reported [2]. It also showed 44.1–92.7% identity at the amino acid level with previously reported plant MAPKs. The OsMAPK33 cDNA was 1549 bp long and encoded a polypeptide of 370 amino acids with a predicted molecular mass of 42.5 kDa. This cDNA had the same deduced amino acid sequence as OsMAP3 (AF216317) [2] and OsMAPK2 (AF241166). The OsMAPK33 cDNA was recently annotated as‘OsMPK14’by The Institute for Genomic Research (TIGR) rice MAPK community. Phylogenetic analysis indicated that OsMAPK33
belongs to C group (C2) of MAPK proteins, along with OsMAPK4, Osmsrmk3 and OsMAP2.
Labs working on this gene
1. Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration, Suwon 441–857, Republic of Korea
2. Department of Biomedical Science, Sun Moon University, Asan 336–708, Republic of Korea
3. Soybean Genomics and Improvement Lab, USDA-ARS, Beltsville, MD 20705, USA
4. Department of Biological Sciences, Seoul National University, Seoul 151–742, Republic of Korea
References
- ↑ 1.0 1.1 1.2 Lee S-K, Kim B-G, Kwon T-R, Jeong M-J, Park S-R, Lee J-W, Byun M-O, Kwon H-B, Matthews BF, Hong C-B and Park S-C 2011 Overexpression of the mitogen-activated protein kinase geneOsMAPK33enhances sensitivity to salt stress in rice (Oryza sativaL.).J. Biosci.36 139–151
- ↑ 2.0 2.1 Wen JQ, Oono K and Imai R 2002 Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice. Plant Physiol.1291880–1891
