Os01g0884300
The rice Os01g0884300 was reported as SNAC2 in 2008 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os01g0884300 <=> OsNAC6, ONAC048, NAC48, SNAC2, SNAC2/OsNAC6, OsNAC6/ONAC048, OsSNAC2
Function
- NAC (NAM, ATAF, and CUC) is a plant specific transcription factor family with diverse roles in development and stress regulation.
- SNAC2 is a novel stress responsive NAC transcription factor that possesses potential utility in improving stress tolerance of rice.
Phenotypic analysis
- The SNAC2 gene was over-expressed in japonica rice Zhonghua 11 to test the effect on improving stress tolerance. More than 50% of the transgenic plants remained vigorous when all WT plants died after severe cold stress (4–8°C for 5 days).
- The transgenic plants had higher cell membrane stability than wild type during the cold stress. The transgenic rice had significantly higher germination and growth rate than WT under high salinity conditions.
- Over-expression of SNAC2 can also improve the tolerance to PEG treatment. In addition, the SNAC2-overexpressing plants showed significantly increased sensitivity to ABA. DNA chip profiling analysis of transgenic plants revealed many up-regulated genes related to stress response and adapta- tion such as peroxidase, ornithine aminotransferase, heavy metal-associated protein, sodium/hydrogen exchanger, heat shock protein, GDSL-like lipase, and phenylalanine ammonia lyase.
- Interestingly, none of the up-regulated genes in the SNAC2-overexpressing plants matched the genes up-regulated in the transgenic plants over-expressing other stress responsive NAC genes reported previously.
Expression
- SNAC2 gene was induced by drought, salinity, cold, wounding, and abscisic acid (ABA) treatment.
Subcellular localization
- SNAC2 was proven to have transactivation and DNA-binding activities in yeast and the SNAC2-GFP fusion protein was localized in the rice nuclei.
Labs working on this gene
- National Center of Plant Gene Research (Wuhan), National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China