Os01g0841500
The rice Os01g0841500 was reported as OsMYB3R-2 in 2009 [1] by researchers from China.
Contents
Annotated Information
Figure 2. Phenotype analysis of the T2 generation of OsMYB3R-2 transgenic rice.[1].
Gene Symbol
- Os01g0841500 <=> OsMYB3R-2, OsMYB3R2, MYB3R2
Function
- MYB transcription factors play central roles in plant responses to abiotic stresses.
- OsMYB3R-2 functions in both stress and developmental processes in rice (Oryza sativa).
- OsMYB3R-2 functions in both stress and developmental processes in rice (Oryza sativa).
- OsMYB3R-2 specifically bound to a mitosis-specific activator cis-element, (T/C)C(T/C)AACGG(T/C)(T/C)A, a conserved sequence that was found in promoters of cyclin genes such as OsCycB1;1 and OsKNOLLE2.
Phenotypic analysis
- Over-expression of OsMYB3R-2(Os01g0841500) may significantly improve cold tolerance by mediating the alteration in cell cycle and ectopic expression of stress genes in rice.
- overexpression of OsMYB3R-2 in rice led to higher transcript levels of several G2/M phase-specific genes, including OsCycB1;1, OsCycB2;1, OsCycB2;2, and OsCDC20.1 than those in OsMYB3R-2 antisense lines or wild-type plants in response to cold treatment.
- Flow cytometry analysis revealed an increased cell mitotic index in overexpressed transgenic lines of OsMYB3R-2 after cold treatment.
- Furthermore, resistance to cold stress in the transgenic plants overexpressing OsCycB1;1 was also enhanced. The level of cellular free proline was increased in the overexpressed rice lines of OsMYB3R-2 and OsCycB1;1 transgenic plants compared with wild-type plants under the cold treatment.
- These results suggest that OsMYB3R-2 targets OsCycB1;1 and regulates the progress of the cell cycle during chilling stress.
- OsCPT1, which may be involved in the dehydration-responsive element-binding factor 1A pathway, showed the same transcription pattern in response to cold as did OsCycB1;1 in transgenic rice.
Expression
- Cold treatment greatly induced the expression of OsMYB3R-2, which encodes an active transcription factor.
Labs working on this gene
- Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
- Graduate University of Chinese Academy of Sciences, Beijing 100049, China
- College of Life Sciences, Shandong Agricultural University, Tai’an 271018, China
- National Center for Plant Gene Research, Beijing 100093, China
References
- ↑ 1.0 1.1 Ma Q, Dai X, Xu Y, Guo J, Liu Y, Chen N, Xiao J, Zhang D, Xu Z, Zhang X, Chong K. Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes. Plant Physiol. 2009 May;150(1):244-56. doi: 10.1104/pp.108.133454. PubMed PMID: 19279197; PubMed Central PMCID: PMC2675741.
