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The rice Os01g0841500 was reported as OsMYB3R-2 in 2009 [1] by researchers from China.

Annotated Information

Figure 2. Phenotype analysis of the T2 generation of OsMYB3R-2 transgenic rice.[1].

Gene Symbol

  • Os01g0841500 <=> OsMYB3R-2, OsMYB3R2, MYB3R2


  • MYB transcription factors play central roles in plant responses to abiotic stresses.
  • OsMYB3R-2 functions in both stress and developmental processes in rice (Oryza sativa).
  • OsMYB3R-2 functions in both stress and developmental processes in rice (Oryza sativa).
  • OsMYB3R-2 specifically bound to a mitosis-specific activator cis-element, (T/C)C(T/C)AACGG(T/C)(T/C)A, a conserved sequence that was found in promoters of cyclin genes such as OsCycB1;1 and OsKNOLLE2.

Phenotypic analysis

  • Over-expression of OsMYB3R-2(Os01g0841500) may significantly improve cold tolerance by mediating the alteration in cell cycle and ectopic expression of stress genes in rice.
  • overexpression of OsMYB3R-2 in rice led to higher transcript levels of several G2/M phase-specific genes, including OsCycB1;1, OsCycB2;1, OsCycB2;2, and OsCDC20.1 than those in OsMYB3R-2 antisense lines or wild-type plants in response to cold treatment.
  • Flow cytometry analysis revealed an increased cell mitotic index in overexpressed transgenic lines of OsMYB3R-2 after cold treatment.
  • Furthermore, resistance to cold stress in the transgenic plants overexpressing OsCycB1;1 was also enhanced. The level of cellular free proline was increased in the overexpressed rice lines of OsMYB3R-2 and OsCycB1;1 transgenic plants compared with wild-type plants under the cold treatment.
  • These results suggest that OsMYB3R-2 targets OsCycB1;1 and regulates the progress of the cell cycle during chilling stress.
  • OsCPT1, which may be involved in the dehydration-responsive element-binding factor 1A pathway, showed the same transcription pattern in response to cold as did OsCycB1;1 in transgenic rice.


  • Cold treatment greatly induced the expression of OsMYB3R-2, which encodes an active transcription factor.

Labs working on this gene

  • Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • College of Life Sciences, Shandong Agricultural University, Tai’an 271018, China
  • National Center for Plant Gene Research, Beijing 100093, China


  1. 1.0 1.1 Ma Q, Dai X, Xu Y, Guo J, Liu Y, Chen N, Xiao J, Zhang D, Xu Z, Zhang X, Chong K. Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes. Plant Physiol. 2009 May;150(1):244-56. doi: 10.1104/pp.108.133454. PubMed PMID: 19279197; PubMed Central PMCID: PMC2675741.

Structured Information