Os01g0228600
The rice Os01g0228600 was reported as OsHPR2 in 2014 [1] by researchers from China.
Contents
Annotated Information
Figure 4. Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.[1].
Gene Symbol
- Os01g0228600 <=> OsHPR2,HPR2
Function
- The researchers constructed over-expression of the OsHPR1 gene and RNA interference plants of OsHPR1 and OsHPR2 genes in rice.
- The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both HPR1 and HPR2 were down-regulated in the same line.
- Double deletion of OsHPR1 and OsHPR2 genes significantly reduces plant growth under normal photorespiration condition
- These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice.
Expression
- The expression of HPR1 gene is induced by light.
- The light regulations on HPR1 gene expression and alternative splicing were species-specific.
- Results from RT-PCR, western-blot and enzyme assays showed that HPR1 activity changed significantly in corresponding transgenic lines without any effect on HPR2 activity, which is the same for HPR2.
Subcellular Localization
- In the present study, the subcellular localization of OsHPR2 protein was first predicted to be located in the peroxisome and cytosol respectively.
- The fluorescent pattern of chimeric protein with HPR2 was found to accumulate in the cytosol and on the surface of the nucleus.
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Labs working on this gene
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China,
- The Chinese University of Hong Kong Shenzhen Institute, Shenzhen, China,
- State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China
