Os01g0192000

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Please cite: Jian Sang (2016) Gene: "Os01g0192000" in RiceWiki, available at http://wiki.ic4r.org/index.php?title=Os01g0192000&oldid=271531 (Last update: Jul 01, 2016). [See details]

The rice Os01g0192000 is located at rice chromosome 1, which is involved in delaying leaf senescence in rice. It was reported as OsDOS by Chinese plant molecular biologists in 2006.

Contents

Annotated Information

Figure 1. OsDOS mutant (RNAi) VS. WT(from reference) [1].

Function

  • Leaf senescence is a developmentally programmed degeneration process that constitutes the final step of leaf development and is controlled by multiple developmental and environmental signals.
  • Research shows that OsDOS acts as a novel negative regulator for leaf senescence by integrating developmental cues to senescence signaling cascades including the JA pathway.
  • Scientists propose that OsDOS possibly plays a role at posttranscriptional level in delaying leaf senescence in rice. And the potential targets of OsDOS might be key positive elements that are required to promote the leaf senescence program.


GO asignment(s): GO:0003676, GO:0008270

Wild Type & Mutants

  • RNAi interference of the OsDOS. The RNAi T 1 lines appeared normal during the vegetative growth. However, beginning at the time of late booting stage, slight but consistent leaf yellowing was clearly observed in the RNAi lines and then an accelerated leaf senescence occurred compared with that in the wild-type plants.
  • At the grainfilling stage, striking leaf yellowing appeared in the whole plant, except the flag leaf showing a slightly less yellowing, and the senescence symptom was correlated with the reduction of the expression level of OsDOS.

Figure 2. Gene structure of Os01g0192000
  • Compared with the OsDOS RNAi lines, the wild-type plants stayed much more green.
Figure 3. OsDOS (Overexpression) mutant VS. WT(from reference) [1].
  • Overexpression of the OsDOS. The OsDOS OX lines showed a delay in growth, shorter stature, abnormally developed panicle, deferred heading, and severe sterility.
  • The florets of the OsDOS OX lines was found failed to open or scarcely opened after heading. Further phenotypic analysis showed that defects in anther dehiscence and pollen viability may be responsible for the severe sterility of the OX lines.
  • Together, these results indicated that the ectopic overexpression of OsDOS has a pleiotropic impact on rice development.

Expression Pattern

  • Real-time PCR analysis was performed to examine the expression profile of OsDOS. The OsDOS transcripts were detected throughout the plant, in panicles and leaves with a higher expression at the early stages. the temporal expression profiles of the OsDOS transcripts indicated a down-regulation of the OsDOS gene expression during natural leaf senescence. Down-regulation of the OsDOS expression also occurred during the panicle development process. In addition, the expression level of OsDOS was reduced after pollination
  • RNA in situ hybridization analysis was also conducted to further examine tissue-specific expression patterns of OsDOS. Strong OsDOS expression was detected in the leaf primordium, incipient leaf, and shoot meristem, but weak expression was observed in the elder leaf tissue
Figure 4. Expression patterns of OsDOS(from reference) [1].

Subcellular Localization

  • To examine the subcellular localization of OsDOS, a green fluorescent protein (GFP) reporter gene was fused in frame to the last codon of the OsDOS coding region to produce an OsDOS-GFP fusion protein in transgenic rice plants. And the OsDOS-GFP green fluorescent signal was detected predominantly in the nuclei of the transgenic rice plants. These results indicate that OsDOS is a nuclear-localized protein and presumably functions as a transcript regulator as predicted for other CCCH-type proteins.

Labs working on this gene

  • Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
  • National Centre for Plant Gene Research, Beijing 100080, China
  • Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • College of Life Sciences, Tianjin Normal University, Tianjin 300074, China

References

  1. 1.0 1.1 1.2 Kong Z, Li M, Yang W, Xu W, Xue Y. A novel nuclear-localized CCCH-type zinc finger protein, OsDOS, is involved in delaying leaf senescence in rice. Plant Physiol. 2006 Aug;141(4):1376-88. Epub 2006 Jun 15. PubMed PMID: 16778011

Structured Information

Gene Locus Os01g0192000
Organism Oryza sativa japonica group
Length 1990 bp
Chromosome Chromosome 01
Location Chromosome 01:4949133...4951123
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IC4R-Expression

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Please cite: Jian Sang (2016) Gene: "Os01g0192000" in RiceWiki, available at http://wiki.ic4r.org/index.php?title=Os01g0192000&oldid=271531 (Last update: Jul 01, 2016).
ContributorContribution Score*Edit Count#Summed Edit QuantityΛAveraged Edit QualityLatest Edit TimeEdit Details
Jian Sang4.506145370.9932016-06-30 16:19:25[show]
Contributors with score < 1 are excluded from the authorship.
ContributorContribution Score*Edit Count#Summed Edit QuantityΛAveraged Edit QualityLatest Edit TimeEdit Details
Shoushao Jin0.02912912016-07-01 16:56:15[show]
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