The rice Os01g0104600 was reported as OsDET1 in 2013  by researchers from China.
- Os01g0104600 <=> OsDET1,DET1
- DE-ETIOLATED1 (DET1) was known to be involved in light transduction and morphogenesis in Arabidopsis and tomato.
- OsDET1 mutation in Gc mutant increases Chl content in rice, which might be fundamental for enhanced photoresponsiveness.
- The physiological effect of the mutated OsDET1 (OsDET1M) on enhanced Chl content was confirmed by genetic transformation.
- The phenotypic trait in Gc mutant was dominant and thus a construct harboring OsDET1M coding sequence and the 3402-bp upstream promoter sequence of the start codon (pOsDET1M) was transformed into Z11, which has similar pigment level and leaf color with Zhenshan 97B (O. sativa indica).
- Fifteen independent transgenic lines were confirmed using GUS staining and PCR amplification (data not shown) and 13 primary lines (T0) displayed dark-green leaf phenotype.
- Further analysis of these 13 transgenic lines demonstrated that, compared to the control Z11, the leaf color phenotype appeared dark-green (Fig. 2a–c), and Chl (Chl a, Chl b, and total Chl) levels of the leaf were enhanced significantly and restored to the levels of that of Gc mutant upon transformation with complementary vector of pOsDET1M::OsDET1M (Fig. 2d and e).
- OsDET1 expression is under circadian control
- OsDET1-GFP fusion protein in onion epidermal cells showed that OsDET1 localized to the nucleus.
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Labs working on this gene
- Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, 174 Shazheng Street,Shapingba District, Chongqing 400030, China
- Institute of Rice, Chongqing Academy of Agricultural Sciences, Chongqing, China
- Huang J, Qin F, Zang G, Kang Z, Zou H, Hu F, Yue C, Li X, Wang G. Mutation of OsDET1 increases chlorophyll content in rice. Plant Sci. 2013 Sep;210:241-9. doi: 10.1016/j.plantsci.2013.06.003. Epub 2013 Jun 13. Erratum in: Plant Sci. 2014 Jan;214:13. PubMed PMID: 23849131.