File:The OsMADS1-DGR fusion protein is functional on induction in transgenic rice.jpg

A, Transfer DNA segment in the construct PUbi: OsMADS1-DGR; P35S:OsMADS1amiR. The 35S promoter drives transcription of an amiRNA targeting the 39 UTR of endogenous OsMADS1 transcripts. The maize ubiquitin cis-elements drive the expression of OsMADS1 translational fusion with the partial rat glucocorticoid receptor (DGR) domain. LB, Left border; RB, right border. B, Awild-type floret. C to E, Floret phenotypes in PUbi:OsMADS1-DGR; P35S:OsMADS1amiR transgenics grown in noninductive mock conditions. F to K, Scanning electron microscopy of epidermal cellular features in glumes, lemma, and palea in wild-type and mock-treated PUbi:OsMADS1-DGR; P35S : OsMADS1amiR lines. L and M, Florets of PUbi:OsMADS1-DGR; P35S:OsMADS1amiR plants grown in inductive conditions (dexamethasone treated). All floret organ phenotypes are rescued. N, Cellular features of rescued palea shown in L. O, Conversion of empty glumes to a lemma/ palea-like organ in M, an overexpression phenotype seen in occasional spikelets. gl, Empty glumele; il, internal lemma; le, lemma; pa, palea. Bars = 1 mm in B to E and L to M and 100 mm in F to K and N to O. P, Western blot with nuclear extracts from leaves of PUbi:OsMADS1-DGR; P35S:OsMADS1amiR lines to detect OsMADS1-DGR protein before induction (lane 1) and after dexamethasone induction (lane 2).