File:Figure 2.JPG
Map-based cloning of d50 gene. (a) Fine mapping of d50 locus. The d50 was located in the 43-kb region between CD5 and RB2. The number indicates recombinants identified from F2 mapping plants. Predicted gene-coding regions are indicated by gray arrows, and the D50 gene is indicated by a black arrow. (b) Schema of D50 gene. The D50 gene contains 11 exons (boxes) separated by 10 introns (lines). Predicted protein-coding regions are indicated by black boxes, the 5-phosphatase domain by blue boxes, and the WD repeats by red boxes. Mutation points of each F71 mutant allele are shown. (c) Complementation of F71 phenotypes by D50 gene. F71 plants introduced D50 cDNA under the 2-kbp promoter region exhibit normal plant height (left), whereas F71 plants introduced the empty vector maintain a dwarf phenotype (right). (d) Cross-sections of transgenic F71 internodes stained by the Mäule reaction. Transgenic F71 plants containing the D50 gene (left) have normal parenchyma cells, whereas plants containing the empty vector (right) exhibit strong staining of the Mäule reaction. (e) DNA gel blot analysis of d12 mutant (m) and its parent cultivar,Yukara (W), using two probes within D50-coding region. The d12 exhibits no signal, indicating the D50-coding region is completely lost from the d12 genome. Bars: 10 cm (c)and 100 mm (d).
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 00:53, 11 June 2014 | 605 × 627 (59 KB) | Leilei Yang (talk | contribs) | Gene isolation and complementation test. (A) High-resolution linkage and physiological map of the OsIAA13 locus. The vertical bars represent molecular markers, and the numbers of recombinant plants are indicated below the linkage map. (B) Structure of the | |
12:03, 10 June 2014 | 452 × 624 (52 KB) | Horatioguo (talk | contribs) | Map-based cloning of d50 gene. (a) Fine mapping of d50 locus. The d50 was located in the 43-kb region between CD5 and RB2. The number indicates recombinants identified from F2 mapping plants. Predicted gene-coding regions are indicated by gray arrows, and |
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