File:Fig-2 mutant insertion for OsTCP1.jpg

Fig-2:Isolation of Tos17insertional mutant of the OsTPC1. (A) The structure of the OsTPC1gene and insertion site of Tos17in the OsTPC1 gene of the mutant line (NF1041). The exon and intron regions of OsTPC1are presented as closed and open bars, respectively. (B) A schematic representation of OsTPC1mRNA and the location of the deletion in the Ostpc1mutant. The approximate locations of primer sequences are shown by arrows. (C) PCR analysis of OsTPC1mRNA in Ostpc1and wild type. PCR products generated by using primers exon11P and P2, which amplify the mRNA region derived fromthe splicing of exon 11 to exon 21. (D) Yeast complementation analysis using Ostpc1cDNA. Square, K927 (cch1)/pYES2; circle, K927 (cch1)/pYES2-OsTPC1; triangle, K927 (cch1)/pYES2-Ostpc1. Vertical bars represent SE (n= 3)