AB462324

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Annotated Information

Function

The recently cloned blast resistance (R) gene Pi-km protects rice crops against specific races of the fungal pathogen Magnaporthe oryzae in a gene-for-gene manner.[1]High-resolution genetic mapping and sequencing of the gene region in the Pikm-containing cultivar Tsuyuake narrowed down the candidate region to a 131-kb genomic interval[2]

常规信息

Pik-m 是稻瘟病抗性位点Pik 上的一个主效抗病等位基因,供体是Tsuyuake

基因的发现与命名

Kiyosawa 报道Minehikari 中含有一个与Pi-k 连锁的抗性基因Pi-m,但后来多个试验表明Pi-m 与Pi-k 等位,于是将Pi-m 重新命名为Pi-km (Kiyosawa, 1978)。

基因的定位

Pi-km 初步定位于水稻第11 染色体长臂近末端区域,SSR 标记RM254 和RM144 之间,遗传距离分别是13.4cM 和1.2cM;通过生物信息学分析,发展新的分子标记,最终精细定位在BAC克隆OSJNBa0036K13 内的84kb区间内(Li et al., 2007)。

基因克隆与生物学功能分析

序列分析和遗传互补实验表明,Pi-km 是由两个紧密连锁的具有独立功能NBS-LRR类基因(Pikm1-TS 和Pikm2-TS)组成。其中,Pikm1-TS 包含有62bp 5'-UTR、3432bp 编码区、2个内含子(119bp, 2769bp)和190bp 3'-UTR;Pikm2-TS 与Pikm1-TS 不同源,包含109bp 5'-UTR、3066bp 编码区、1个内含子(163bp)和283bp 3'-UTR(Ashikawa et al., 2008)。 Pikm1-TS 和Pikm2-TS 的编码产物均是NBS-LRR类抗病蛋白,长度分别为1143aa和1021aa。他们在结构上有些小的差异:Pikm1-TS 氨基端发现有nT基序,属于CC结构,而Pikm2-TS 虽也发现有nT基序,但未发现有CC结构;Pikm1-TS 羧基端有非LRR结构,而Pikm2-TS 没有(Ashikawa et al., 2008)。

备注

NBS-LRR 类抗病蛋白

Expression

Evolution

Labs working on this gene

Please input related labs here.

References

1. Stefano Costanzo;Yulin Jia,Sequence variation at the rice blast resistance gene Pi-km locus: Implications for the development of allele specific markers.Plant Science, 2010, 178(6): 523-530

2.Ikuo Ashikawa;Nagao Hayashi;Hiroko Yamane;Hiroyuki Kanamori;Jianzhong Wu;Takashi Matsumoto;Kazuko Ono;Masahiro Yano, Two Adjacent Nucleotide-Binding Site–Leucine-Rich Repeat Class Genes Are Required to Confer Pikm-Specific Rice Blast Resistance.Genetics, 2008, 180(4): 2267-2276

3. Luo-Ye Li;Ling Wang;Jin-Xue Jing;Zhen-Qi Li;Fei Lin;Li-Fei Huang;Qing-Hua Pan,The Pikm gene, conferring stable resistance to isolates of Magnaporthe oryzae, was finely mapped in a crossover-cold region on rice chromosome 11. Molecular Breeding, 2007, 20(2): 179-188

4. Shigehisa Kiyosawa,Identification of Blast-Resistance Genes in Some Rice Varieties.Japanese Journal of Breeding, 1978, 28(4): 287-296

Structured Information

Gene Name

AB462325

Description

3458 bp mRNA linear PLN 20-MAR-2009.

Version

AB462325.1 GI:207367331

Length

3458 bp

Definition

Oryza sativa Japonica Group Pikm2-TS mRNA for NBS-LRR class disease

           resistance protein, complete cds.
Source

Oryza sativa Japonica Group (Japanese rice) ORGANISM Oryza sativa Japonica Group

           Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
           Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
           clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome

Chromosome 11

Location

Chromosome11:110..3175

Sequence Coding Region

110..3175

Expression

GEO Profiles:AB462325

Genome Context

{{{GCID}}}

Gene Structure

{{{GSID}}}

Coding Sequence

{{{CDNA}}}

Protein Sequence

<aaseq>MELVVGASEATMKSLLGKLGNLLAQEYALISGIRGDIQYINDEL

                    ASMQAFLRDLSNVPEGHSHGHRMKDWMKQIRDIAYDVEDCIDDFAHRLPQDSISDAKW
                    SFLLTKIYELWTWWPRRVIASNIAQLKVRAQQIADRRSRYGVNNPEHLDSSSSARTRA
                    VNYEIAEYQVTSPQIIGIKEPVGMKTVMEELEVWLTNPQAENGQAVLSIVGFGGVGKT
                    TIATALYRKVSEKFQCRASVAVSQNYDQGKVLNSILSQVSNQEQGSSTTISEKKNLTS
                    GAKSMLKTALSLLRGNCICQPENDGNPDNTPIRLQETTDDDQNPRKLEQLLAEKSYIL
                    LIDDIWSAETWESIRSILPKNNKGGRIIVTTRFQAVGSTCSPLETDRLHTVDFLTDDE
                    SQNLFNTSICESKIRKDSNKVDEQVPEEIWKICGGLPLAIVSMAGLVACNPRKACCDW
                    SKLCKSLFPEQETPLTLDGVTRILDCCYNDLPADLKTCLLYLSIFPKGWKISRKRLSR
                    RWIAEGFANEKQGLTQERVAEAYFNQLTRRNLVRPMEHGSNGKVKTFQVHDMVLEYIM
                    SKSIEENFITVVGGHWQMTAPSNKVRRLSMQSSGSNRGSSTKGLNLAQVRSLTVFGNL
                    NHVPFHSFNYGIIQVLDLEDWKGLKERHMTEICQMLLLKYLSIRRTEISKIPSKIQKL
                    EYLETLDIRETYVRDLPKSIVQLKRIISILGGNKNTRKGLRLPQEKSKKPIKNPSPQG
                    KTKEPAKKGFLSQEKGKGAMKALRVLSGIEIVEESSEVAAGLHQLTGLRKLAIYKLNI
                    TKGGDTFKQLQSSIEYLGSCGLQTLAINDENSEFINSLGDMPAPPRYLVALELSGKLE
                    KLPKWITSITTLNKLTISVTVLRTETLEILHILPSLFSLTFAFSLSAAKQDQDIIKDI
                    LENNKLDSDGEIVIPAEGFKSLKLLRFFAPLVPKLSFLDKNAMPALEIIEMRFKDFEG
                    LFGIEILENLREVHLKVSDGAEAITKFLVNDLKVNTEKPKVFVDGIVTA</aaseq>
Gene Sequence

<dnaseqindica>1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341# 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421# aggacacgga aattctcagc aggttcgcgc ttgatctgaa ctgtctgtgg ctcatactct cttgtgcttg caacagctga aagttgcagt gagaagtaca gagaacaaga tggagttggt ggtaggtgct tccgaagcca ccatgaaatc tctcttgggc aagctgggca atcttctagc ccaggagtat gctctcatca gcggtatccg tggtgacatc cagtacatca atgacgagct tgccagcatg caggccttcc tccgtgatct cagcaacgtg ccagagggtc acagtcatgg ccaccggatg aaggactgga tgaagcagat ccgagacatc gcctatgatg ttgaggactg tatcgatgac tttgcccacc gcctccctca ggattccatc agcgatgcca aatggtcctt cctactcaca aaaatctatg aactatggac atggtggcca cgtcgtgtga ttgcttccaa cattgcccaa ctcaaggtac gggcacaaca gatcgcagat cgacgtagta gatacggagt gaacaaccca gaacaccttg acagtagcag cagtgccagg acccgtgctg tcaattacga aattgctgag tatcaggtca caagccctca gatcattggt ataaaggagc ctgtggggat gaagacggtc atggaggagc ttgaggtttg gttaactaat cctcaagctg aaaatgggca agctgttctg tccatagtcg gttttggagg tgtgggaaag actaccattg ccacagcatt gtacagaaaa gtcagtgaaa aatttcagtg ccgggcatca gtagctgtgt ctcagaacta tgaccaaggc aaagtcctca atagtattct gagtcaagtc agcaatcagg agcagggcag cagcacaaca attagtgaga aaaagaacct cacctcaggc gctaagagca tgttgaagac agccctgtca ctgctcagag gtaattgtat atgtcagcca gaaaatgatg gaaaccctga

     taatacacca atcaggctgc aggaaacaac ggacgatgat caaaacccca gaaaactgga
     acagctcctg gccgaaaaga gttatatcct cttgattgat gacatttggt ctgccgaaac
     atgggagagt atcagatcga ttttgcctaa aaataataaa ggcggtagaa taatagtgac
     tacaagattt caagctgttg gttcaacatg ctcccctctt gaaactgatc gtttgcatac
     agttgatttt ctcaccgatg acgagtccca aaacttattc aatacaagta tttgtgaatc
     aaagataaga aaagatagca acaaagtaga cgagcaagtc cctgaggaaa tatggaaaat
    atgtggggga ttgcctttgg ccatagtcag catggctggt cttgtcgcct gcaacccaag
    gaaagcctgc tgcgattgga gtaaactttg caaatcatta tttccagagc aagaaactcc
     tcttaccctc gatggtgtta caaggatact ggattgttgt tacaatgatt tgcctgcgga
     tctgaagact tgcttattgt acttgagtat atttccgaag ggttggaaaa ttagtaggaa
     acgtttgtcc cggcgatgga tagctgaagg ttttgctaat gagaagcaag ggttaaccca
     ggaaagagtt gcagaggcat actttaatca actcacaaga aggaacttag tacgtcccat
    ggagcatggc agcaatggga aggtaaaaac gtttcaagtt catgacatgg ttcttgaata
     catcatgtcc aaatcaatcg aagagaattt tattactgtg gttggtggac actggcagat
     gactgcacca agcaataaag tccgtcgact gtcgatgcaa agcagtggat ccaatcgtgg
    aagttcaaca aaaggcctga acttggctca agtgagatca ctgacggtgt ttgggaacct
    gaaccatgtg ccattccatt cattcaacta tgggataata caggtgctgg atcttgagga
     ctggaagggt ttgaaagaga gacatatgac ggagatatgt caaatgcttt tactcaagta
     tttgagcatc cgacgaacag aaatttccaa aattccctcc aagattcaga aacttgagta
     cttggaaact cttgacataa gggagacata tgtcagggac ctgcctaagt caatagtcca
    gctaaaacgg atcattagca tacttggagg gaataaaaac acacggaagg ggctgaggtt
     gcctcaagaa aaaagtaaga agccaattaa aaacccgtcg cctcaaggaa aaacaaagga
     gcccgcaaag aaaggattct tatcccaaga aaaaggtaaa ggcgcaatga aagcactccg
     tgtactgtca gggattgaga ttgttgagga atcatcagaa gtagctgcag gccttcatca
     gttgacaggg ctaaggaagc ttgccatata caagctcaat ataacaaagg gtggtgatac
     cttcaaacaa ttacagtcct ccattgagta ccttggcagc tgtggtctgc agactctggc
     catcaatgat gagaattctg aatttatcaa ctcactgggc gacatgcccg cgcctccaag
     atatcttgtc gcccttgagc tgtctggcaa gttggagaag ctacccaagt ggatcaccag
     catcactact ctcaacaagc taaccatatc tgtaacagtt cttaggactg aaactttgga
     gatcctccac attttacctt cattgttttc cctcaccttc gccttttcac ttagtgcagc
     gaagcaggat caggacataa taaaggacat ccttgagaat aataaattgg acagtgatgg
     ggaaatcgtc attccagctg aaggattcaa gagtcttaag ctgcttcgct tctttgcacc
     tttagtgccg aagctcagct ttttggacaa gaatgcaatg ccagcactcg aaatcattga
     aatgcggttt aaagacttcg aaggtctatt tggcatcgaa atccttgaaa atctccgtga
     ggtgcatctc aaagttagtg atggggcaga agcaataacc aagttccttg taaatgattt
     gaaggttaat actgagaaac caaaagtatt tgttgatggc atcgtcactg catgagaagt
     aaaattgctg caaatcggag aacttaccaa tcatctgagg cttcccctct attattactc
     tcttagaata tattgttatt attgctcacc ttgcaaaata aaatagggat ggcatagcat
     attgctacaa cgtaccatgg ttccatcata gttgatttca cttgtcatta cagtgtctgt
     tcagttgtgt tttctattaa taaaagggag atctccgcaa gaaaccatta ttatacttat
     attcggttat tgaactctat aaatgatggg attgctat</dnaseqindica>
External Link(s)

NCBI Gene:AB462325, [1]