AB013449

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Pib is a member of the NBS-LRR class of plant disease resistance genes.

Annotated Information

Function

Pib is a member of the NBS-LRR class of plant disease resistance genes.Pib is a member of a small gene family. The Pib gene encodes a polypeptide of 1251 amino acids; it contains an NBS region and C-terminal LRRs, but no distinct transmembrane domain. Thus, the Pib gene belongs to the NBS-LRR class of plant disease resistance genes and is predicted to encode a cytoplasmic protein. We speculate that an interaction between the Pib gene product and a ligand specified by the avirulence genes of incompatible rice blast races may occur in the cytoplasm, as predicted for other NBS-LRR plant disease resistance genes. It is not clear how many members there are in the Pib family. Two members included c23 (Pib) mapped onto chromosome 2 , and the third, cE (PibH8) was mapped onto chromosome 8. The Pib locus comprises a cluster of two homologues contained within the 80 kb region covered by the cosmid contig.

Expression

 There has been only one other report of induced R gene expression. The mRNA for the Xa1 gene for resistance bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) was detected from rice leaves 5 days after wounding or inoculation with either compatible or incompatible strains of Xoo, but was not detected in intact leaves .Pib was expressed at a low level in intact leaves, and increased in expression at 12 and 24 HAI following inoculation with both incompatible and compatible (data not shown) races of rice blast and returned to a low level again at 96 HAI.  Like many environmental stress response genes, the Pib gene was induced by altered environmental conditions, such as altered temperatures and darkness. The significance of this induction response to the altered environmental conditions is not clear. The expression of Pib gene remains to be investigated under other environmental stresses, such as wounding, cold treatment, dehydration, etc.

Labs working on this gene

1.International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines 2.Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan 3.Indonesian Center for Rice Research (ICRR), JL. Raya Muara No. 25A Ciapus Bogor, Subang, West Java, Indonesia 4.Agricultural Genetics Institute, Conhue, Tuliem, Hanoi, Vietnam 5.Faculty of Agricultural Sciences, University of Burundi, BP 2940 Bujumbura, Burundi

References

1.Wang Z X, Yano M, Yamanouchi U, et al. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine‐rich repeat class of plant disease resistance genes[J]. The Plant Journal, 1999, 19(1): 55-64. 2.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b

 Theoretical and Applied Genetics, 1997, 94(2): 170-176

3.Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)

 Plant Breeding, 2010, 129(6): 670-675

4.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b

 Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13

2.Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)

 Plant Breeding, 2010, 129(6): 670-675

3.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b

 Theoretical and Applied Genetics, 1997, 94(2): 170-176

Structured Information

Template:Oryza sativa Japonica Group (Japanese rice)